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Figure 7 | Veterinary Research

Figure 7

From: Betanodavirus-like particles enter host cells via clathrin-mediated endocytosis in a cholesterol-, pH- and cytoskeleton-dependent manner

Figure 7

RBS enters GB and SSN-1 cells via CME. VLP entry assay, IFA and fluorescent microscopy were performed on ten cell lines and RBS can only enter GB and SSN-1 as indicated in VLP + panels. To identify the endocytic pathway, GB and SSN-1 cells were pretreated with CPZ for 1 h. VLP entry assay, IFA and fluorescent microscopy were performed and RBS entry was dramatically decreased in VLP +/CPZ + samples. RBS (green) in cells were visualized by IFA and cell nuclei (blue) were stained by DAPI. The bars indicate 25 μm.

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Veterinary Research

ISSN: 1297-9716