Figure 4

In vitro growth kinetics of BTV1, 8 and 15 on VERO and BPAEC cells. VERO cells (A) and BPAEC (B) were infected at a multiplicity of infection (M.O.I.) of 0.05 and supernatants collected at 8, 24, 48, and 120 h post infection (hpi). Viruses were the same as used for single infection (BTV1 and BTV15) and BTV8 was the same as in the BTV8 successive infection experiment. Supernatants were then titrated on VERO cells by end-point dilution and the virus titres expressed as log₁₀ TCID₅₀/mL. Three different assays were performed independently, each time involving the three serotypes, tested on the different cell lines. Standard deviations are displayed as error bars.