Figure 3

Validation of antisense RNA (asRNA) expressed in vivo and in plate culture. A Electrophoresis gel of four asRNA amplified by RT-PCR showing amplification results for RNA purified from plate culture with RT step (P+), plate culture without RT step (P-), 2 h in vivo sample with RT (V+) or without RT step (V-). Genomic DNA was included as the positive control (C+) and water as the negative control (C-). B Venn diagram showing overlapping Gene Ontology (GO) enriched terms for up- and down-regulated asRNA or sense RNA at 5% FDR.