Figure 6From: Evaluation of biological safety in vitro and immunogenicity in vivo of recombinant Escherichia coli Shiga toxoids as candidate vaccines in cattle Gene transcription in bovine iIEL and surface marker expression on bovine MDM incubated for 6 h with rStx WT or rStx mut . (A) Relative amounts of gene transcripts for IL-4 harboured by PHA-P-stimulated (2.5 mg/mL) bovine iIEL normalized to the transcription of the housekeeping gene GAPDH. Vector control cultures were used as reference and set to 100% (dashed line). (B) Expression (i.e., mean fluorescence intensity; MFI) of CD14 on bovine CD77+ MDM relative to vector control cultures defined as 1.0 (dashed line). Data is depicted as means ± standard deviations from 5 independent experiments each. Statistical analysis was performed as described in legend to Figure 2. Significance levels were defined as p ≤ 0.01 [**], and p ≤ 0.05 [*].Back to article page