Figure 4From: Evaluation of biological safety in vitro and immunogenicity in vivo of recombinant Escherichia coli Shiga toxoids as candidate vaccines in cattle Effect of recombinant Shiga toxins and toxoids on CD77 expression by bovine CD8β + PBMC. Cells were incubated in culture medium containing 5 μg/mL PHA-P and rStxWT or rStxmut as indicated. After four days of incubation cells were submitted to immunolabelling and flow cytometry analysis. Percentages of CD8β+ PBMC co-expressing CD77 (events in the upper right quadrant) are given in the upper right corner of the dot plots. PBMC incubated in the presence of PHA-P and vector control were used as control (upper graph).Back to article page