Figure 6

Expression of phosphorylated ERK1/2- and p38 MAPK in sporozoite-exposed PMN and influence of their inhibition on parasite-triggered NETosis. Total proteins of sporozoite-exposed PMN and non-exposed controls were analyzed by SDS/PAGE and immunoblot technique using specific antibodies against non-phosphorylated and phosphorylated ERK1/2 (A) and p38 MAPK (B). Respective bands were densitometrically analyzed. Each bar represents the arithmetic means ± SEM of three PMN donors. Differences were regarded as significant at a level of p ≤ 0.05 compared to negative controls. (C) NET formation in presence of ERK 1/2 (UO126) and p38 (SB 203580) MAPK inhibitors. Stimulation with zymosan served as a positive control; PMN in plain medium as a negative control.