Immunogenicity and efficacy of fowlpox-vectored and inactivated avian influenza vaccines alone or in a prime-boost schedule in chickens with maternal antibodies

Table 4 Results of the vaccination-challenge study (Study 4)

Group	MDA	Vaccine		H5N8 HI titers^a		H5N9 HI titers	Protection^b	Oropharyngeal shedding^c
Group	MDA	D0	D14	D14	D28	D28	(MDT)	2 Dpc	4 Dpc
1	-	-	-	ND^d	0/10 (2.0 ± 0.0)	0/10 (2.0 ± 0.0)	0/10 (2.0)	10/10 (7.8 ± 0.3)	ND
2	-	vFP89	-	10/10 (3.7 ± 0.8)	10/10 (5.5 ± 1.2)	8/10 (2.9 ± 0.6)	1/10 (3.4)	10/10 (6.3 ± 1.3)	5/5 (6.6 ± 0.7)
3	FP	vFP89	inH5N9	2/9 (2.3 ± 0.7)	9/9 (8.1 ± 1.6)	9/9 (7.6 ± 1.8)	9/9	6/9 (4.6 ± 0.7)	4/9 (4.0 ± 0.5)
4	FP	-	inH5N9	ND	9/9 (5.1 ± 1.6)	9/9 (5.8 ± 0.7)	5/9 (7.3)	7/9 (4.9 ± 0.9)	4/9 (4.6 ± 1.0)
5	H5N9	-	-	ND	0/9 (2.0 ± 0.0)	0/9 (2.0 ± 0.0)	0/9 (2.0)	9/9 (7.3 ± 0.1)	ND
6	H5N9	vFP89	-	ND	5/9 (2.9 ± 0.9)	0/9 (2.0 ± 0.0)	0/9 (2.7)	9/9 (7.1 ± 0.5)	1/1 (7.7)
7	H5N9	vFP89	inH5N9	0/9 (2.0 ± 0.0)	9/9 (5.8 ± 1.0)	9/9 (4.3 ± 1.1)	7/9 (7.0)	6/9 (4.5 ± 0.6)	7/9 (4.6 ± 1.3)
8	H5N9	-	inH5N9	0/9 (2.0 ± 0.0)	4/9 (3.2 ± 1.6)	6/9 (3.7 ± 1.7)	4/9 (5.0)	8/9 (5.0 ± 1.0)	7/8 (5.3 ± 1.1)

Results of serology, protection and oropharyngeal viral shedding in chicks with or without FP or H5N9 MDA vaccinated or not with vFP89 and/or inH5N9 and challenged at D28 with an HPAI H5N1 Indonesian isolate (A/chicken/West Java-Subang/29/2007).
^aNumber of positive birds (HI titer ≥ 3 log2)/total; the mean HI titer ± standard deviation is given between brackets.
^bNumber of birds protected against mortality/total; the mean death time (MDT) is given between brackets.
^cNumber of positive birds (≥3.8 log10 EID50-equivalent)/total; the mean viral RNA load ± standard deviation is given in log10 EID50-equivalent between brackets; Dpc, day post-challenge.
^dND means not done.

ISSN: 1297-9716