Figure 3From: Duck MDA5 functions in innate immunity against H5N1 highly pathogenic avian influenza virus infectionsEffects of different functional domains of Muscovy duck MDA5 on the chicken IFN-β promoter. The pCAGGS-MDA5, pCAGGS-MDA5ΔRD, pCAGGS-MDA5ΔCARD, pCAGGS-MDA5ΔCARD + ΔRD, pCAGGS-MDA5-CARD or the empty plasmid was cotransfected with pGL3-chIFNβ-Luc and pTK-RL plasmid in DEF cells. Dual-luciferase assays were performed after 36 h.Back to article page