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Table 1 Bovine viral diarrhoea virus (BVDV) detection and pathology in rabbits at day 5 after challenge by intravenous route (IV) or nebulizer (N)

From: Experimental infection of rabbits with bovine viral diarrhoea virus by a natural route of exposure

 

BVDV RT-PCRa

Virus isolation

GALT depletionc

Animal

Buffy coat

Ileum

Appendix

Spleen

Ileum

 

IV5

neg

+

+

+

+

moderate-severe

IV6

+

+

+

+

+

mild-moderate

IV7

+

+

+

+

+

moderate-severe

IV8b

neg

neg

neg

neg

neg

none

N5

neg

+

+

+

not done

indeterminatec

N6

neg

neg

+

+

not done

mild-moderate

N7

neg

neg

neg

+

not done

mild-moderate

N8b

neg

neg

neg

neg

not done

indeterminatec

  1. aRT-PCR for viral RNA detection was performed as described in the materials and methods. In each case, 1 μL of total RNA was used in assays specific for BVDV type 1 and for genomic β-actin. Assays with Ct for BVDV and for actin less than 40 were considered positive (+), while assays with Ct for BVDV greater than 40.1 and Ct for actin less than 40 were considered negative (neg).
  2. bnegative control animals.
  3. cGut associated lymphoid tissue (GALT) depletion was assessed by histopathology as described in the materials and methods section. In group N, GALT depletion could not be determined clearly in two animals due to autolysis.