Figure 6
BPIV3, BHV-1-GFP and BRSV-GFP infection of PCLS before or after neuraminidase treatment. PCLS were first incubated for 1 h with or without 100 mU/PCLS neuraminidase type V and then infected with BPIV3 or BHV-1-GFP with 105 FFU/mL for 2 h or with BRSV-GFP for 3 h. At 2 dpi, the slices were fixed and in case of BPIV3 stained for virus antigen (green). Cilia were visualized by staining against β-tubulin (red). Scale bar = 100 μm.