Figure 3From: The new French 2010 Rabbit Hemorrhagic Disease Virus causes an RHD-like disease in the Sardinian Cape hare (Lepus capensis mediterraneus)Phylogenetic analysis of rabbit hemorrhagic disease virus (RHDV) isolates and RHDV2 isolates identified in Italian hares and rabbits. For the phylogenetic analysis of the VP60 sequences, the neighbor-joining method was applied based on the Kimura 2-parameters using the software package MEGA5 [20]. The European brown hare syndrome virus (EBHSV) strain BS89 (GenBank accession number: X98002) was used as an outgroup to root the tree. Bootstrap probability values above 70% for 1000 replicate runs are indicated at the nodes. Twenty selected sequences representing previously described RHDV genogroups (G1-G6, [18]), four sequences of RCV-like isolates, and the sequences of the RHDV2 isolates identified in France in 2010 (strain 10–32) and in Italy (strains Ud11 and Tn12-1) were included. The GenBank accession numbers of these strains are indicated. The RHDV sequences from rabbits isolated before spring 2011 (accession numbers: KC595270 and KC345614) belong to genogroup 5, whereas those from rabbits and hares collected after this period (accession numbers: KC345611, KC345612, KC345613, and JX106023) belong to the genogroup RHDV2.Back to article page