Figure 6

Infection dynamics of MDM from resistant or susceptible deer, infected with MAP in vitro. (A) Infection rate of macrophages from crossbred red deer of a resistant or susceptible genotype. MDM were isolated from crossbred red deer and cultured for 7 days on slide flasks. At day 7 of MDM culture, the cells were infected with MAP (MOI of 10:1) for 24 or 48 h and the resulting MAP infection rate was determined by auramine staining. The data are expressed as percentages of MAP-infected cells (mean ± SEM) and at least 75 cells per slide were counted, n = 13 (6 resistant and 7 susceptible). (B) Numbers of MAP bacilli per infected macrophage from crossbred red deer of a resistant or susceptible genotype. The data are expressed as percentages of MAP-infected macrophages that contain different numbers (≤ 10, 10 – 20, ≥ 20) of MAP bacilli at 24 and 48 hours after infection (mean ± SEM), n = 13 (6 resistant and 7 susceptible).