Figure 5
Western blot analysis of proteinase K treated PrPScin C57BL/6 and VM mice detected with Sha31 antibody. (a) Compares the molecular profile of different classical scrapie strains as defined by IP and LP analysis following stabilisation in C57BL/6 mice: Lane 2, ovine classical scrapie; Lane 3, ME7 in C57BL/6 control; Lanes 5–6, ME7 mice; Lanes 7–8, 87A mice; Lanes 9–10, 221C mice; Lanes 11–12, VLA-C1 mice; Lane 14, unchallenged C57BL/6 mouse; Lanes 1, 4 and 13, molecular mass markers. (b) Compares the molecular profile of different classical scrapie strains as defined by IP and LP analysis following stabilisation in VM mice: Lane 2, ovine classical scrapie; Lane 3, 87V in VM control; Lanes 5–6, ME7 mice; Lanes 7–8, 87V mice; Lanes 9–10, VLA-V1 mice; Lane 12, unchallenged VM mouse; Lanes 1, 4 and 11, molecular mass markers. (*) indicates that the sample originated in study 2. (c) Glycoform profiles of challenged VM mice: comparing relative band intensity of di- versus non-glycosylated PrPSc. Results represent molecular profiles for individual animals taken from two experiments: squares, experiment 1; circles, experiment 2.