The role of glycoprotein H of equine herpesviruses 1 and 4 (EHV-1 and EHV-4) in cellular host range and integrin binding

Azab, Walid; Zajic, Lara; Osterrieder, Nikolaus

doi:10.1186/1297-9716-43-61

Veterinary Research

Table 1 Olignucleotide primers used in this study

From: The role of glycoprotein H of equine herpesviruses 1 and 4 (EHV-1 and EHV-4) in cellular host range and integrin binding

Primer	Product	Sequence
P1	EHV-1gH	tat ggatcc atgttacaaccgtatcgaaa^a
P2	EHV-1gH	ata gggccc ttactcataactcaataaca^a
P3	EHV-4gH	tat ggatcc atgtcacaaccgtatctaaa^a
P4	EHV-4gH	ata gggccc ttactcagagtttaataaca^a
P5	Kan-1	ggcgcggccgc cggttcgcatgttatcctcaaggatgacgacgataagtaggg^a,b
P6	Kan-1	ccggcggccgc gccaacagcaaaaaatagcacaaccaattaaccaattctgattag^a,b
P7	Kan-4	tattctaga aagccgctaccaaacgcggtaggatgacgacgataagtaggg^a,b
P8	Kan-4	ctttctaga ataagcgtacacgcttttcacaaccaattaaccaattctgattag^a,b
P9	gH1-deletion	gtggctgtacattaacttgggaatcattacttccgcgatcacaaatatcccgtgtgttgtaggatgacgacgataagtaggg^b
P10	gH1-deletion	attgtctaacatggggggtaacaacacacgggatatttgtgatcgcggaagtaatgattccaaccaattaaccaattctgattag^b
P11	gH4-deletion	atccagtggttgtatattgggaataaatactgctgcgattacacaaacaatgtctagtgtaggatgacgacgataagtaggg^b
P12	gH4-deletion	ctgtttacacgcaatacaacacactagacattgtttgtgtaatcgcagcagtatttattccaaccaattaaccaattctgattag^b
P13	gH4-Kan	aagatataccgtggctgtacattaacttgggaatcattacttccgcgatcatgtcacaaccgtatctaaa
P14	gH4-Kan	tcgcacaaatattgtctaacatggggggtaacaacacacgggatatttgtttactcagagtttaataaca
P15	gH1-Kan	tactcgaggtatccagtggttgtatattgggaataaatactgctgcgattatgttacaaccgtatcgaaa
P16	gH1-Kan	actcgtatactgtttacacgcaatacaacacactagacattgtttgtgtttactcataactcaataaca
P17	gH^440A	ccagtttgacgttgcacaatcccagattgagaaaatagtgG cagatatcaacgtggaggccaggatgacgacgataagtaggg^b,c
P18	gH^440A	tacatcggtttgcgcaattcggcctccacgttgatatctgC cactattttctcaatctgggcaaccaattaaccaattctgattag^b,c
P19	Primers for sequencing	tgtgacctggattcatttag
P20		aacaattttacgcgtaatat
P21		ttgtgctcttaaatcattta
P22		gccgcattcccgtttatagc
P23		taatgtcaaaaaatctttta
P24		gtttacgtactcagcgatgg
P25		ccatacgtgatataactgat
P26		gtgaggataacatgcgaacc
P27		atgacaatttggagccgttt
P28		tatgcaggacctatctacaa
P29		actataggctttgctatatt

^aRestriction enzyme sites are given in lower case bold letters; sequences in italics indicate additional bases which are not present in the EHV-1 or -4 sequence.
^bUnderlined sequences indicate the template binding region of the primers for PCR amplification with pEPkan-S.
^cUpper case bold letters indicate the nucleotides that were mutated.

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ISSN: 1297-9716

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