Days post 1st inoculation (pi) | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Experiment | Assay | 7 | 14 | 21 | 28 | 35 | 42 | 49 | 56 | 63 | 70 | 77 | 84 |
1 | Viral isolation (titre) | 14/14 | 11/14† | 4/11 | 1/11 | 0/11 | 0/8 | 0/8 | 0/8 | 0/8 | 0/8 | 0/8 | 0/8 |
3.1 ± 0.6 | 2.9 ± 0.5 | 2.3 ± 0.2 | 2.3 ± 0.0 | - | - | - | - | - | - | - | - | ||
nRT-PCR | 14/14 | 11/14 | 4/11 | 1/11 | 0/11 | 0/8 | 0/8 | 0/8 | 0/8 | 0/8 | 0/8 | 0/8 | |
2 | Viral isolation (titre) | 8/8 | 8/8 | 7/7††* | 7/7* | 7/7* | 3/7* | 3/7* | 1/7* | 0/7 | 0/7 | 0/7 | 0/7 |
3.7 ± 0.3 | 3.4 ± 0.5 | 3.2 ± 0.8 | 2.6 ± 0.5 | 2.2 ± 0.2 | 2.0 ± 0.3 | 2.0 ± 0.3 | 1.8 ± 0.0 | - | - | - | - | ||
nRT-PCR | N.D. | N.D. | N.D. | N.D. | N.D. | 4/7 | 4/7 | 3/7 | 0/7 | 0/7 | 0/7 | 0/7 |