Figure 3

IHC and histopathology for the cohabitant group. Immunostaining of heart tissue sections and graphical presentation of the IHC- and histopathological scores from the cohabitant group. (A) Blood clot in heart 8 wpi: PRV antigen detected with σ1-antibodies in numerous leukocyte-like cell (arrow). Error bar 25 μm. (B) Heart ventricle 10 wpi: A high number of immunostained cardiomyocytes in the spongy layer. Section stained with σ1-antibodies. Error bar 100 μm. (C) Heart ventricle 12 wpi: Scattered immunostained cardiomyocytes (arrow) detected with σ1-antibodies in the inner part of compact layer. Inflammation response in the outer part of the compact layer seen in the top half of the picture. Error bar 100 μm. (D) The graph illustrates the IHC staining with Anti-σ1 and the histopathological changes at each time of sampling (Wpi). IHC staining scores (left Y-axis) are indicated for leukocyte-like cells (blue) and cardiomyocytes (red). Histopathological scores (right Y-axis) are presented for epicardial- (gray) and ventricular changes (black). All bars represent mean values (+SD) for each group (n = 5).