Figure 6From: Differential modulation of cytokine, chemokine and Toll like receptor expression in chickens infected with classical and variant infectious bursal disease virusRelative gene expression at mRNA level, of proinflamatory cytokine, iNOS, and chemokine mRNA in IBDV-infected bursa. At PIDs 3, 5 and 7, bursal mononuclear cells were isolated from cIBDV, vIBDV and virus-free control chickens and examined for IL-6 (A), iNOS (B), IL-8 (C), and MIP-α (D) gene expression by qRT-PCR. Results are shown as transcription of the target gene relative to housekeeping gene 28S. The data are expressed as fold change expression in infected chickens over virus-free control. The values represent the mean ± SE of 4 pools of 3 bursa each at designated PID. #Statistically significant differences between control and vIBDV- or cIBDV-infected groups (p < 0.05). *Statistically significant differences between vIBDV- and cIBDV-infected groups (p < 0.05).Back to article page