Novel gene expression responses in the ovine abomasal mucosa to infection with the gastric nematode Teladorsagia circumcincta

Table 3 Details of gene sequences, and likely function, selected for further analysis

Functional Category	Gene Name	Clone/probe ID on KN511 cDNA array	EMBL acc. no.	Assay
Cytotoxicity	GNLY	C0009264H11.P1KAM13F	FE028690	W1
	CatC	sh1-aoo11_l0.s2	DY479012	W2
	GZMA	KN511_9486f02	FE037399	W2
	GZMB	KN511_9253n09	FE021442	W1
	GZMH	KN511_9260m05	FE026184	W2
Mucus composition	CLCA1	020502OAPP1012080HT OAPP	EE748540	W1
	ITLN1	-^a	AM087961	W2
	ITLN2	KN511_9264a24.p1kaM13F	EF521881	W2
	ITLN3	-^a	AM888394	W2
Heat shock response	HSPA8	KN511_9479o03	DY491193	W1
	HSPCA	KN511_9253c04	FE020983	W2
	ST1	KN511_9486e08	FE037366	W1
Proinflammatory response	PLA2G2A	KN511_9482g22	FE034658	W1
	CF1	KN511_9257m10	FE024140	W1
	CCL2	KN511_9477e07	FE031007	W1
Tissue remodelling	MMP13	KN511_9487m11.p1kM13F	AY091604	W1
	MMP23	sh1-bjd04_l0.s2	DY483383	W2
	CST3	KN511_9476g20	FE030675	W1
Digestion	LZM1A	KN511_9487n22.p1kM13F^b	M32492	W1
	LZM4A	KN511_9487n22.p1kM13F^b	M32497	W1
Unknown	MALAT	KN511_9484l14	FE036272	W1
"Housekeeping" genes	ATPase	KN511_9488b23.p1kM13F	X02813	W1/W2
	RW1	KN511_9261h17	FE026635	W1/W2
	TM57	KN511_9488i21	FE030125	W1/W2

The EMBL accession no. for the sequences used to design multiplex competitive qRT-PCR assays (Sequenom^® MassARRAY^® System) is given, together with the representative probe on the ovine cDNA array. The two multiplex competitive qRT-PCR assays were designated W1 and W2 as indicated.
^aOnly ITLN2 was represented on the arrays.
^bThese cDNA probes would not be able to distinguish between LZM1A, 3A and 4A.

ISSN: 1297-9716