Figure 3
From: RACK1 mediates NLRP3 inflammasome activation during Pasteurella multocida infection
RACK1 promotes P. multocida-induced formation of ASC specks. Cells were transfected with si-RACK1 to knockdown RACK1 and pcDNA3.1-RACK1 plasmid to overexpress RACK1 for 48 h. Then, cells were infected for 24 h as described above. Finally, immunofluorescence staining was performed to detect NLRP3 and ASC specks in RACK1-knockdown cells (A) and RACK1-overexpressing cells (B). White arrows represent the colocalization of ASC and NLRP3. The images are representative of three independent experiments. The bar in each microscopic image indicates 50 μm.