Figure 6From: Modulation of the host Th1 immune response in pigeon protozoal encephalitis caused by Sarcocystis calchasiPCR detection of S. calchasi DNA in brains of pigeons with specific nested primer pairs targeting the ITS1 region. All brains of pigeons of group A (no. 2–9) and pigeons of group B (no. 10–12, 15, L60 and L74) except for no. 14 were positive. Five reference animals (r1-r5) used for RT-qPCR as well as negative controls and two processing controls were negative.Back to article page