Figure 2
From: Identification of the ovine mannose receptor and its possible role in Visna/Maedi virus infection
LTR promoter activity in ovine skin fibroblasts (OSF). OSF were transfected with pGL4/U3-KV1772 (clone containing VMV LTR), pGL4.13 containing SV40 promoter (as positive control), or pGL4 for basal activity (as negative control). Transfected cells were treated with ConA, ConA and VMV or medium and the LTR activity was measured 24 h pi using the Luciferase reporter system (Promega). Firefly luciferase activity was normalized to renilla luciferase activity. Data are expressed as luciferase units/ng total protein of each sample. Values are the mean ± SE of assays performed.